Linda Archer

Linda Archerarcherl@ufl.edu

Equipment

  • Molecular Biology
  • Gel electrophoresis
  • Thermocyclers
  • PCR hoods
  • Biosafety cabinets
  • Microcentrifuges (refrigerated & non)
  • Mg scales
  • ELISA plate washer
  • Real time PCR (FAST) machine
  • Incubator (non CO2 and CO2)
  • Centrifuge (3000 xg max)
  • Vacuum centrifuge

Training or Skills

  • DNA and RNA extraction methodologies
  • PCR (Polymerase Chain Reaction) techniques
  • Real Time PCR methods to quantify viral RNA/DNA levels in extracted samples
  • Gel Electrophoresis
  • Purification of nucleic acid fragments from the gel
  • Primer design to create new primers used in all PCR methods
  • Consensus PCR methods used to determine the presence of a virus belonging to a larger category of viruses such as an order, family or genus
  • Documentation of experimental research results Flow cytometry
  • Immunohisto chemistry
  • Cell culture routines
  • Perform cloning techniques