Rachel Wilkes

rsanford@ufl.edu

Equipment

  • Elisa reader Dynex (visible light)
  • Dual head microscope
  • Microscope with old nikon coolpix camera
  • Delphin Trans epidermal water loss reader
  • PCR machine used for RT reactions only
  • 9600 PCR machine
  • StepOne Plus AB Biosystem Real time PCR machine
  • Nuwind refrigerated centrifuge
  • Harrier refrigerated centrifruge
  • Minus 80 freezer
  • Amerex shaking water bath
  • Analytical balance
  • 2 steel kennels-multiple med dog travel kennels
  • Old style projector for slides
  • Lab Vision Autostainer

Training or Skills

  • Western Blotting
  • ELISA
  • Immunohistochemistry
  • Animal handling (dogs, cats, marine organisms)
  • PCR
  • Molecular cloning
  • RNA and DNA extraction
  • Next Generation Sequencing (Illumina, Ion Torrent)
  • Library Preparation for NGS
  • Bioinformatics
  • Cryostat brain sectioning and mounting